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Merck

ZRB1798

Sigma-Aldrich

Anti-GLS Antibody, clone 1D16 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.43

Biologische Quelle

rabbit

Qualitätsniveau

Rekombinant

expressed in HEK 293 cells

Konjugat

unconjugated

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

1D16, recombinant monoclonal

Beschreibung

1D16 Clone

Produktlinie

ZooMAb® learn more

Form

lyophilized

Mol-Gew.

calculated mol wt 73.46 kDa
observed mol wt ~60 kDa

Aufgereinigt durch

using Protein A

Speziesreaktivität

rat, human, mouse

Verpackung

antibody small pack of 25 μL

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Erweiterte Validierung

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Methode(n)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotyp

IgG

Epitopsequenz

N-terminal

Protein-ID-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Grünere Alternativprodukt-Kategorie

Versandbedingung

ambient

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... GLS(2744)

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 1D16 is a ZooMAb® Rabbit recombinant monoclonal antibody that detects kidney mitochondrial form of human glutaminase.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of human Glutaminase (GLS).

Anwendung

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GLS in Rat brain tissue lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected GLS in lysates from Mouse brain tissue and from PC12, SH-SY5Y, and HepG2 cells.

Affinity Binding Assay:: A representative lot of this antibody bound GLS with a KD of 2.4 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLS in SH-SY5Y cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GLS in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Zielbeschreibung

Glutaminase kidney isoform, mitochondrial (UniProt: O94925; also known as EC:3.5.1.2, GLS, K-glutaminase, L-glutamine amidohydrolase) is encoded by the GLS (also known as GLS1, KIAA0838) gene (Gene ID: 2744) in human. GLS catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. It is primarily expressed in the brain and kidney and plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate, and maintaining acid-base balance in the kidney. Following its synthesis, it is found in cytoplasm and in the mitochondria. The 74-kDa cytosolic precursor is reported to translocate into the mitochondria with the help of a mitochondrial transit peptide (aa 1-54) and is processed to yield the mature 68 kDa (aa 55-669) and 65-kDa (aa 73-669)subunits. During normal acid-base balance, the kidney extraction of plasma glutamine is low, however, during metabolic acidosis, about one third of the plasma glutamine is extracted and metabolized in a single pass through kidneys. Three isoforms of glutaminase have been described in humans that are produced via alternate splicing. Isoforms 1 and 3 are activated by phosphate, while the isoform 3 lacks catalytic activity. Isoform 1 is highly expressed in brain and kidney, but is not detected in liver. On the other hand, isoform 3 is highly expressed in astrocytoma, ganglioglioma, ependymoma. Lower levels of isoform 3 are reported in placenta, lung, pancreas, and kidney, but it is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle. Mutations in GLS gene are known to cause developmental and epileptic encephalopathy 71 (DEE71)s that is characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Rumping, L., et al. (2019). JAMA Neurol. 76(3); 342-350; Elgadi, KM., et al. (1999). Physiol. Genomics 1(2); 51-62).

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstituierung

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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