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Merck

ZRB1652

Sigma-Aldrich

Anti-CACNA2D1 Antibody, clone 1K4 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(e):

Voltage-dependent calcium channel subunit alpha-2/delta-1;Voltage-gated calcium channel subunit alpha-2/delta-1

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Rekombinant

expressed in HEK 293 cells

Konjugat

unconjugated

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

1K4, recombinant monoclonal

Beschreibung

recombinant, expressed in HEK 293 cells

Produktlinie

ZooMAb® learn more

Form

lyophilized

Mol-Gew.

calculated mol wt 124.57 kDa

Aufgereinigt durch

using Protein A

Speziesreaktivität

human

Speziesreaktivität (Voraussage durch Homologie)

monkey, bovine

Verpackung

antibody small pack of 25 μL

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Erweiterte Validierung

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Methode(n)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

Isotyp

IgG

Epitopsequenz

N-terminus

Protein-ID-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Grünere Alternativprodukt-Kategorie

Versandbedingung

ambient

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 1K4 is a Rabbit recombinant monoclonal antibody that specifically detects Voltage-dependent calcium channel subunit alpha-2/delta-1 (CACNA2D1). It targets an epitope within 22 amino acids from the N-terminal, extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal, extracellular domain of human Voltage-dependent calcium channel subunit alpha-2/delta-1 (CCNA2D1).

Anwendung

Quality Control Testing

Evaluated by Immunocytochemistry in U-251 MG cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CACNA2D1 in U-251 MG cells.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CACNA2D1 in human skeletal muscle.

Affinity Binding Assay: A representative lot of this antibody bound CACNA2D1 with a KD of 7.9 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Zielbeschreibung

Voltage-dependent calcium channel subunit alpha-2/delta-1 (UniProt: P54289; also known as Voltage-gated calcium channel subunit alpha-2/delta-1, CACNA2D1) is encoded by the CACNA2D1 (also known as CACNL2A, CCHL2A, MHS3) gene (Gene ID: 781) in human. CACNA2D1 is a dimeric protein formed by disulfide-linked 2 and chains. It regulates calcium current density and activation/inactivation kinetics of the calcium channel and is shown to play an important role in excitation-contraction coupling. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-1073), a transmembrane domain (aa 1074-1094), and a short cytoplasmic tail (aa 1095-1103). It also has a MIDAS-like motif (aa 259-263), which is present in its VWFA domain (aa 253-430). The MIDAS-like motif is shown to bind divalent metal cations and is required to promote trafficking of the a-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch. Five different isoforms of CACNA2D1 have been described that are produced by alternative splicing. Isoform 1 is expressed in skeletal muscle, isoform 2 is expressed in the central nervous system, and isoforms 3, 4, and 5 are expressed in the aorta. Isoforms 2, 4, and 5 are also observed in neuroblastoma cells. Mice bearing point mutations in the CACNA2D1 gene display reduced response to pregabalin and an abnormal central nervous system synaptic transmission. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Vergult, S., et al. (2015). Eur. J. Hum. Gen. 23(5); 628-632; Brust, PF., et al. (1993). Neuropharmacology. 32(11); 1089-1102).

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstituierung

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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