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Merck

ZRB1448

Sigma-Aldrich

Anti-Recoverin Antibody, clone 1K10 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(e):

Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.43

Biologische Quelle

rabbit (recombinant)

Qualitätsniveau

Rekombinant

expressed in HEK 293 cells

Konjugat

unconjugated

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

1K10, monoclonal
recombinant monoclonal

Produktlinie

ZooMAb® learn more

Form

lyophilized

Mol-Gew.

calculated mol wt 23.13 kDa
observed mol wt 20 kDa

Speziesreaktivität

human, bovine

Verpackung

antibody small pack of 25 μL

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Erweiterte Validierung

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Methode(n)

affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

Isotyp

IgG

UniProt-Hinterlegungsnummer

Grünere Alternativprodukt-Kategorie

Versandbedingung

ambient

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... RCVRN(5957)

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spezifität

Clone 1K10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Recoverin.

Immunogen

His-tagged full-length human recombinant Recoverin.

Anwendung

Anti-Recoverin, clone 1K10 ZooMAb, Cat. No. ZRB1448, is a recombinant rabbit monoclonal antibody that specifically targets Recoverin and is tested for use in Affinity Binding Assay, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Recoverin in human retina tissue sections.

Immunofluorescence Analysis: 1:500 dilution from a representative lot detected Recoverin in human retina tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant recoverin protein with a KD of 6.0 x 10-9 in an affinity binding assay.

Immunocytochemistry Analysis: 1:100 dilution of this antibody detected Recoverin in Y79 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Zielbeschreibung

Recoverin (UniProt P35243; also known as Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known as RCV1) gene (Gene ID 5957) in human. The photoreceptor protein recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins. It acts as a calcium sensor and regulates phototransduction of cone and rod photoreceptor cells. It modulates light sensitivity of cone photoreceptor in dark and dim conditions. It improves rod photoreceptor sensitivity in dim light and mediates response of rod photoreceptors to facilitate detection of change and motion in bright light. In response to high Ca2+ levels induced by low light levels, it prolongs RHO/rhodopsin activation in rod photoreceptor cells by binding to and inhibiting G-protein-coupled receptor kinase 1 (GRK1)-mediated phosphorylation of RHO/rhodopsin. Recoverin is composed of four EF-hand domains (aa 25-60, 61-96, 97-132, 147-182), with the second and third EF-hand regions mediating low- and high-affinity calcium binding, respectively. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstituierung

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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