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Merck

ZRB1270

Sigma-Aldrich

Anti-mGluR5 Antibody, clone 1F11 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.43

Biologische Quelle

rabbit

Qualitätsniveau

Rekombinant

expressed in HEK 293 cells

Konjugat

unconjugated

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

1F11, recombinant monoclonal

Beschreibung

recombinant, expressed in HEK 293 cells

Produktlinie

ZooMAb® learn more

Form

lyophilized

Mol-Gew.

calculated mol wt 132.47 kDa
observed mol wt ~155 kDa

Speziesreaktivität

rat

Speziesreaktivität (Voraussage durch Homologie)

mouse, bovine, human

Verpackung

antibody small pack of 25 μL

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Erweiterte Validierung

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Methode(n)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotyp

IgG

Epitopsequenz

C-terminus

Protein-ID-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Grünere Alternativprodukt-Kategorie

Versandbedingung

ambient

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spezifität

Clone 1F11 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Metabotropic glutamate receptor 5 (mGluR5). It targets an epitope within 23 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids the C-terminal region of human Metabotropic glutamate receptor 5 (mGluR5).

Anwendung

Quality Control Testing

Evaluated by Western Blotting in Rat brain microsome.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected mGluR5 in Rat brain microsome.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound mGluR5 peptide with a KD of 2.0 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected mGluR5 in E18 rat cortical cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected mGluR5 in rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This rabbit recombinant monoclonal Anti-mGluR5, clone 1F11 ZooMAb® Rabbit Monoclonal, Cat. No. ZRB1270 is used in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry and Affinity Binding Assay, for the detection of mGluR5.

Zielbeschreibung

Metabotropic glutamate receptor 5 (UniProt: P41594; also known as mGluR5) is encoded by the GRM5 (also known as GPRC1E, MGLUR5) gene (Gene ID: 2915) in human. Glutamate receptors can be grouped into ionotropic receptors and metabotropic receptors. Excitotoxic effects of glutamate analogs are primarily achieved through the activation of the ionotropic receptors. Glutamate can also activate metabotropic receptors that couple GTP-binding proteins to intracellular second messengers. These receptors are distributed throughout the central nervous system and are located at synaptic as well as extra-synaptic sites in neurons and glia. Metabotropic receptors contain a large extracellular N-terminal domain known as the Venus flytrap domain (VFD) that contains the glutamate-binding site and has two lobes that sit one atop the other and bind glutamate in a cleft between the two lobes. The VFD dimer can exist in open-open, open-closed, or in closed-closed state. The open-open state is an inactive conformation and is stabilized by antagonists. At least eight metabotropic glutamate receptors (mGluR1-8) have been reported. They are classified into three groups based on their sequence homology, agonist pharmacology, and coupling to intracellular transduction mechanisms. The mGluR1 and mGluR5 belong to group I and these receptors are coupled to post-synaptic inositol phosphate metabolism. They couple to Gq/G11 and activate phospholipase Cβ that hydrolyzes phosphoinositides to generate inositol 1, 4, 5-trisphosphate (IP3) and diacyl- glycerol (DAG), both of which participate in the activation of protein kinase C. Activation of Group I receptors leads to depolarization and increases neuronal excitability. mGluR1 is present at high levels in the cerebellum and thalamus and mGluR5 is found in superficial cortical layers and the olfactory bulb. The activation of mGluR1 generates a single spike in Ca2+ concentrations, whereas mGluR5 activation leads to Ca2+ oscillations via phosphorylation/dephosphorylation cycles. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstituierung

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1


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