V1514

Anti-VE-Cadherin (CD144) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-CD144, Anti-Cadherin-5, Anti-Vascular Endothelial Cadherin
• MDL-Nummer: MFCD01633075
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~130 kDa
• Speziesreaktivität: human
• Methode(n): immunohistochemistry (frozen sections): 1:100 using acetone-fixed sections of human tonsil; western blot: 1:500 using whole extract of human umbilical vein endothelial cells (HUVEC).
• UniProt-Hinterlegungsnummer: P33151
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CDH5(1003)

Beschreibung

Allgemeine Beschreibung

Cadherin 5 (CDH5), also known as cadherin 144 (CD144), is encoded by the gene mapped to long arm of human chromosome 16. The encoded protein is expressed in endothelial cells, but it absent in normal epithelium.

VE-Cadherin (also designated vascular endothelial cadherin, cadherin-5, and cluster of differentiation 144 (CD144)) is a non-type I or II cadherin that is expressed on endothelial cells of all blood and lymphatic vessels, where it organizes intercellular adherens junctions.

Immunogen

synthetic peptide corresponding to amino acids 91-110 of human VE-cadherin (CD144).

Anwendung

Anti-VE-Cadherin (CD144) antibody produced in rabbit has been in following studies:

• immunofluorescence imaging
• western blotting
• to weaken cell-cell junctions in human umbilical vein endothelial cells (HUVECs)
• immunohistochemistry

Biochem./physiol. Wirkung

Cadherin 5 (CDH5)/CD144 plays an essential role in various cellular processes, such as regulation of cell adhesion, cell proliferation, cell survival, cell shape, cell motility. Additionally, it is also involved in the regulation of signaling pathways and transcriptional gene regulation. CD144/VE-cadherin is a core component of endothelial adherens junction, which plays a vital role in angiogenic sprouting by enhancing cell migration and cell elongation. CD144 expressed in glioblastoma multiforme (GBM) facilitates the transdifferentiation of glioblastoma stem like cells (GSCs) to endothelial cells and form blood vessels, under hypoxia condition. VE-cadherin induces endothelial cell-cell adhesion at adherens junctions (AJs). CD144 gene knockout results in loss of cell polarity and modification in vascular lumenal structure. CD144 inhibits the synthesis of CDH2 (also known as N-cadherin) in the endothelium.

VE-cadherin (CD144) interacts with β-catenin, plakoglobinand Adherens junction protein (p120ctn).

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable