T3450

Thiolutin

from Streptomyces luteosporeus, ≥95% (HPLC)

• Synonym(e): Farcinicin, N-(4,5-Dihydro-4-methyl-5-oxo-1,2-dithiolo[4,3-b]pyrrol-6-yl), Propiopyvothine
• Empirische Formel (Hill-System): C₈H₈N₂O₂S₂
• CAS-Nummer: 87-11-6
• Molekulargewicht: 228.29
• MDL-Nummer: MFCD07370147
• UNSPSC-Code: 12352105
• PubChem Substanz-ID: 329826766
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: Streptomyces luteosporeus
• Qualitätsniveau: 200
• Assay: ≥95% (HPLC)
• Form: solid
• Löslichkeit: DMSO: 0.90 - 1.10 mg/ml, clear, yellow
• Wirkungsspektrum von Antibiotika: fungi
• Wirkungsweise: enzyme | inhibits
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• SMILES String: CCC(=O)NC1=C2SSC=C2N(C)C1=O
• InChI: 1S/C9H10N2O2S2/c1-3-6(12)10-7-8-5(4-14-15-8)11(2)9(7)13/h4H,3H2,1-2H3,(H,10,12)
• InChIKey: UGZYFXMSMFMTSM-UHFFFAOYSA-N

Beschreibung

Anwendung

Thiolutin has been used as a polymerase II inhibitor:

• to study its effects on yeast cells to calculate transcript half-life
• to study its effects on transcription during germination in budding yeast
• to study its effects on cell adhesion in zebrafish

Biochem./physiol. Wirkung

Sulfur-containing antibiotic, which is a potent inhibitor of bacterial and yeast RNA polymerases. It was found to inhibit in vitro RNA synthesis directed by all three yeast RNA polymerases (I, II, and III). Thiolutin is also an inhibitor of mannan and glucan formation in Saccharomyces cerevisiae and used for the analysis of mRNA stability. Studies have shown that thiolutin inhibits adhesion of human umbilical vein endothelial cells (HUVECs) to vitronectin and thus suppresses tumor cell-induced angiogenesis in vivo.

Angaben zur Herstellung

Thiolutin dissolves in DMSO at 0.90 - 1.10 mg/ml to yield a clear, yellow solution.

Sicherheitshinweise

• Piktogramme: GHS06
• Signalwort: Danger
• H-Sätze: H300
• P-Sätze: P264 - P270 - P301 + P310 - P405 - P501
• Gefahreneinstufungen: Acute Tox. 2 Oral
• Lagerklassenschlüssel: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges