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T0951
Anti-Tet Repressor (TETR) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(e):
Anti-Tetracycline Repressor Protein
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About This Item
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Konjugat
unconjugated
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
buffered aqueous solution
Mol-Gew.
antigen ~22 kDa by calculation
Speziesreaktivität
human
Methode(n)
indirect ELISA: suitable
western blot: 1:1,000
Versandbedingung
dry ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Verwandte Kategorien
Allgemeine Beschreibung
Tet Repressor (TETR) is homodimer comprising a C-terminal helix-turn-helix motif (HTH) and N-terminal DNA-binding domain,. It also has a C-terminal tetracycline-binding and dimerization (TBD) domain.
Immunogen
Anti-Tet Repressor (TETR) reacts with amino acid residues 31-49 (AQKLGVEQPTLYWHVKNKR) of 22 kDa Tet repressor protein
Anwendung
Anti-Tet Repressor (TETR) antibody produced in rabbit has been used in western blotting.
Biochem./physiol. Wirkung
The Tet system is employed in conditional gene expression studies in eukaryotic cells. TetR ( can be used for such applications in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator. TetR interaction with antibiotic tetracyclin decreases its binding affinity to DNA.
Physikalische Form
Solution of 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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The TetR family of transcriptional repressors
Ramos JL, et al.
Microbiology and Molecular Biology Reviews, 69(2), 326-356 (2005)
E Pook et al.
European journal of biochemistry, 258(3), 915-922 (1999-02-17)
We isolated five monoclonal antibodies (mAbs) made against tetracycline repressor (TetR), one against the TetR tetracycline complex (Tc) and two against the TetR-tet operator (tetO) complex. The epitopes of the anti-TetR mAbs are localized in the alpha-helix-turn-alpha-helix motif (HTH), at
FOXO1 is an essential regulator of pluripotency in human embryonic stem cells
Zhang X, et al.
Nature Cell Biology, 13(9), 1092-1092 (2011)
W Hillen et al.
Annual review of microbiology, 48, 345-369 (1994-01-01)
Tetracycline-resistance determinants encoding active efflux of the drug are widely distributed in gram-negative bacteria and unique with respect to genetic organization and regulation of expression. Each determinant consists of two genes called tetA and tetR, which are oriented with divergent
The application of Tet repressor in prokaryotic gene regulation and expression
Bertram R and Hillen W
Microbial Biotechnology, 1(1), 2-16 (2008)
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