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Merck

SRP6557

Sigma-Aldrich

Thrombin Active from human plasma

≥95% (SDS-PAGE), recombinant, lyophilized

Synonym(e):

Activated Factor IIa

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.32

product name

Thrombin Active from human plasma, ≥95% (SDS-PAGE)

Biologische Quelle

human

Assay

≥95% (SDS-PAGE)

Form

lyophilized

Wirksamkeit

>3000 units/mg

Mol-Gew.

37 kDa

Verpackung

pkg of 1000 units
pkg of 250 units

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

human ... F2(2147)

Allgemeine Beschreibung

Thrombin enzyme (activated factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. It is primarily associated with blood clotting.

Biochem./physiol. Wirkung

Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. It also induces inflammation. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.

Physikalische Form

Lyophilized from a buffer composed of 20 mM Bis-Tris, 150 mM NaCl and 0.1% PEG 8000, pH 6.5.

Rekonstituierung

A working stock solution can be prepared by adding 1 mL buffered saline, pH 7.4 (ex. 25 mM HEPES, 150 mM NaCl, 0.1% PEG 8000, pH 7.4). Further dilution should be made in buffer containing a suitable stabilizing agent such as 0.1%-1% Prionex, BSA, or PEG.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3


Analysenzertifikate (COA)

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Bruce AC and Peirce SM
Microcirculation (New York, N.Y. : 1994), 19(2), 143-154 (2012)
Tobias Strittmatter et al.
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Structure of the thrombin complex with triabin, a lipocalin-like exosite-binding inhibitor derived from a triatomine bug.
Fuentes-Prior P
Proceedings of the National Academy of Sciences of the USA, 94(22), 11845-11850 (1997)
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