SRP6241

VAP-1 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE)

• Synonym(e): AOC3, Copper Amine Oxidase, HPAO, Membrane Primary Amine Oxidase, SSAO, Vascular Adhesion Protein-1
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in CHO cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol-Gew.: ~80 kDa
• Verpackung: pkg of 10 μg; pkg of 50 μg
• Verunreinigungen: <1 EU/μg endotoxin (LAL test)
• UniProt-Hinterlegungsnummer: Q16853
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... VAP1(8639)

Beschreibung

Allgemeine Beschreibung

VAP-1 (vascular adhesion protein 1) is also called as semicarbazide-sensitive amine oxidase. The VAP-1 gene is mapped to human chromosome 17q21.31.

VAP-1 is a type II membrane cell adhesion protein belonging to the copper/topaquinone oxidase family. It is primarily expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. VAP-1 can catalyze the oxidative deamination of low molecular weight amines, and plays an important role in the migration of lymphocytes to inflamed tissue. Inhibition of VAP-1 can protect against inflammation related damage to certain injured tissues. Additionally, VAP-1 can function as a significant prognostic marker for certain cancers and cardiovascular diseases. Recombinant VAP-1 is a mixture of monomeric and disulfide linked homodimeric forms of a 737 amino acid polypeptide corresponding to amino acids 27 to 763 of the VAP-1 precursor.

Biochem./physiol. Wirkung

VAP-1 (vascular adhesion protein 1) catalyzes an oxygen-dependent reaction involving the conversion of primary amines to aldehyde, ammonia and hydrogen peroxide. Overexpression of VAP-1 is observed in chronic inflammatory diseases such as chronic renal failure, diabetesand autoimmune disorders. VAP-1 mediates the interaction between leukocytes and endothelium in response to inflammation.

Physikalische Form

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable