SRP4515

Artemin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

• Synonym(e): ART, ARTN, EVN, NBN
• MDL-Nummer: MFCD03099559
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• Form: lyophilized
• Wirksamkeit: 2-5 ng/mL ED₅₀ (it′s ability to stimulate the proliferation of human SH-SY5Y cells)
• Mol-Gew.: ~24 kDa
• Verpackung: pkg of 20 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Verunreinigungen: endotoxin, tested
• NCBI-Hinterlegungsnummer: NM_057090
• UniProt-Hinterlegungsnummer: Q5T4W7
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... ARTN(9048)

Beschreibung

Allgemeine Beschreibung

Human Artemin, a neurotrophic factor, belongs to the GDNF family. Artemin is a survival factor for sensory and sympathetic neurons in culture. Recombinant human Artemin is a 24.2 kDa, disulfide-linked homodimer formed by two identical 113 amino acid subunits. The gene encoding this protein is localized on human chromosome 1p34.1.

Biochem./physiol. Wirkung

Artemin (ART/ARTN) is a survival factor for sensory and sympathetic neurons in culture. It has a role in promoting survival of neurons. ART is also involved in the differentiation and development of the central and peripheral nervous systems. The protein promotes axonal projection and migration in sympathetic neurons. Artemin restores neural damage and diminishes neuropathic pain. It has been associated with various carcinomas.

Physikalische Form

Lyophilized from 10 mM Sodium Citrate pH 4.5 plus 150 mM NaCl.

Angaben zur Herstellung

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Rekonstituierung

Reconstitute in water to a concentration of 0.1-0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Recontitute in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/mL. This stock solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable