SRP4171

IL-15 from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): IL 15, IL-T, Interleukin 15
• MDL-Nummer: MFCD00675504
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Rekombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: ~13.3 kDa
• Verpackung: pkg of 10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Verunreinigungen: endotoxin, tested
• NCBI-Hinterlegungsnummer: P48346
• UniProt-Hinterlegungsnummer: P48346
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Il15(16168)

Beschreibung

Allgemeine Beschreibung

IL15 (interleukin 15) protein is a short-chain α-helical bundle type 1 T cell-stimulating cytokine. It is a pleiotropic cytokine belonging to the cytokine receptor γ chain family, and is expressed by dendritic cells (DCs), epithelial cells, macrophages, monocytes, and fibroblasts. IL15, along with its receptor IL15Rα, are co-expressed majorly by activated monocytes and DCs (dendritic cells). Recombinant mouse IL-15 is a 13.3 kDa protein containing 115 amino acid residues.

Biochem./physiol. Wirkung

IL15 (interleukin 15) and IL2 are the only two known cytokine till date that bind to T-cells through IL-2Rβ (IL2 receptorβ). It interacts with lymphocytes via the heterotrimeric IL-15 receptor (IL-15R) composed of IL-15Rα, IL-2 receptor beta (IL-2Rβ), and IL-2 receptor gamma (IL-2Rγ). Since, IL-2R and IL-15R have common subunits, it is supposed that IL2 and IL15 perform similar functions, such as induction of lymphocyte activation and proliferation in vitro, and apoptosis of activated lymphocytes (AICD). However, unlike IL2, IL15 mimics the function of IFN I (interferon) by potently and selectively activating memory-phenotype CD44^hi CD8⁺ cells.

Physikalische Form

Lyophilized from 10 mM Tris, pH 8.0.

Rekonstituierung

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 μg/μL. The solution can then be diluted into other aqueous buffers.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable