SRP3299

GDF-5 mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): BMP-14, CDMP-1
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Rekombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Form: lyophilized
• Wirksamkeit: 0.08-0.12 μg/mL
• Mol-Gew.: 27.0 kDa
• Verpackung: pkg of 50 μg
• Verunreinigungen: <0.1 EU/μg endotoxin, tested
• Farbe: white to off-white
• UniProt-Hinterlegungsnummer: P43027
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... GDF5(14563)

Beschreibung

Allgemeine Beschreibung

Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 348 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease.

Biochem./physiol. Wirkung

GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Recombinant murine GDF-5 is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acids.

Physikalische Form

Lyophilized with no additives.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable