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Merck

SRP3242

Sigma-Aldrich

IL-2 aus Ratte

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(e):

Aldesleukin, T-cell growth factor (TCGF)

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.32

Biologische Quelle

rat

Rekombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

Form

lyophilized

Wirksamkeit

≤0.4 ng/mL ED50

Mol-Gew.

15.3 kDa

Verpackung

pkg of 20 μg

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

<0.1 EU/μg endotoxin, tested

Farbe

white to off-white

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

rat ... IL2(116562)

Allgemeine Beschreibung

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues.

Biochem./physiol. Wirkung

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues.

Sequenz

MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAIAQSI ISTM

Physikalische Form

Lyophilized from 10 mM Sodium Citrate, pH 4.0.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

K A Smith
Science (New York, N.Y.), 240(4856), 1169-1176 (1988-05-27)
Interleukin-2 (IL-2), the first of a series of lymphocytotrophic hormones to be recognized and completely characterized, is pivotal for the generation and regulation of the immune response. A T lymphocyte product, IL-2 also stimulates T cells to undergo cell cycle
Nabil Ahmed et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 33(15), 1688-1696 (2015-03-25)
The outcome for patients with metastatic or recurrent sarcoma remains poor. Adoptive therapy with tumor-directed T cells is an attractive therapeutic option but has never been evaluated in sarcoma. We conducted a phase I/II clinical study in which patients with
Lindsey Chudley et al.
Cancer immunology, immunotherapy : CII, 63(11), 1199-1211 (2014-08-20)
Ex vivo ELISPOT and multimer staining are well-established tests for the assessment of antigen-specific T cells. Many laboratories are now using a period of in vitro stimulation (IVS) to enhance detection. Here, we report the findings of a multi-centre panel
Luiza M Araujo et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(9), 2412-2422 (2014-06-10)
HLA-B27/human β2 -microglobulin (hβ2 m)-transgenic (B27-transgenic) rats develop an inflammatory disorder resembling spondyloarthritis, with accumulation of proinflammatory Th17 cells. Because Treg cells and Th17 cells have opposing effects in inflammatory disorders, we sought to determine whether biased expansion of Th17
Eun-Mi Kim et al.
Immunology, 142(3), 421-430 (2014-02-18)
Murine embryonic stem (ES) cell-derived haematopoietic progenitor cells (HPCs) engraft and populate lymphoid organs. In vivo, HPCs engraft across MHC barriers protecting donor-type allografts from rejection. However, the underlying phenomenon remains elusive. Here, we sought to determine the mechanism by

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