SRP0729

PCTK3 (CDK18)/CYCLINY, Active

PRECISIO^® Kinase, recombinant

• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• Form: frozen liquid
• Spezifische Aktivität: 35-55 nmol/min·mg (radiometric)
• Mol-Gew.: ~64 kDa (Cyclin Y); ~76 kDa (PCTK3)
• Konzentration: 0.1 μg/μL
• Hinterlegungsnummer: BC011526; NM_145012
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... CCNY(219771) , CDK18(5129)

Beschreibung

Anwendung

Useful for kinase assay and Western blot.

Biochem./physiol. Wirkung

Full-length recombinant human PCTK3 (CDK18) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK3 or PCTAIRE protein kinase 3 is a member of the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK3 is thought to play a role in terminally differentiated neurons and it contains an N-terminal extension relative to CDC2 ⁽¹⁾. PCTK3 is expressed in a variety of human cell lines and tissues ⁽²⁾. Elevation in the protein levels of PCTAIRE 3 in the temporal cortex of Alzheimer′s disease relative to control brains, have been observed. It is proposed that PCTAIRE 3 is a paired helical filaments-associated kinase that modulates tau phosphorylation.

Rekonstituierung

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Lagerung und Haltbarkeit

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable