SRP0720

SBK1, Active

PRECISIO^® Kinase, recombinant

• UNSPSC-Code: 12352202

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• Form: frozen liquid
• Spezifische Aktivität: 15-25 nmol/min·mg (radiometric)
• Mol-Gew.: ~69 kDa
• Konzentration: 0.05-0.1 μg/μL
• Hinterlegungsnummer: NM_001024401
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... SBK1(388228)

Beschreibung

Anwendung

Useful for kinase assay.

Biochem./physiol. Wirkung

Full-length recombinant human SBK1 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. SBKI or SH3 domain binding kinase 1 is a novel serine/threonine protein kinase structurally related to a Xenopus gastrula-specific protein kinase, Pk9.7 which possessing a consensus sequence for an SH3-binding domain from developing rat brain and it play an important role in signal-transduction pathways related to the control of brain development ⁽¹⁾. SBK1 may predict a broad cellular function, and its dysregulated in certain cancers suggests an involvement of the protein in the pathogenesis of human cancers ⁽²⁾.

Rekonstituierung

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Lagerung und Haltbarkeit

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable