SRP0699

STK9 Protein

recombinant

• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• Form: frozen liquid
• Mol-Gew.: ~84 kDa
• Konzentration: 0.1 μg/μL
• Hinterlegungsnummer: NM_003159
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... STK9(6792)

Beschreibung

Anwendung

Useful for Western blot.

Biochem./physiol. Wirkung

Recombinant human STK9 (CDKL5) (1-498) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. STK9 or CDKL5 is a member of Ser/Thr protein kinase family which has protein kinase activity and is associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). STK9 can control nuclear speckle morphology by regulating the phosphorylation state of splicing regulatory proteins, and may be involved indirectly in pre-mRNA processing, by controlling splicing factor dynamics ⁽¹⁾. The mutations in the STK9 is a major cause of infantile spasms and early epileptic seizures in female patients, and of a later intractable seizure disorder ⁽²⁾.

Rekonstituierung

Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Lagerung und Haltbarkeit

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable