SRP0320

JMJD2b active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

• Synonym(e): KDM4B, lysine (K)-specific demethylase 4B
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥70% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 83 kDa
• Verpackung: pkg of 100 μg
• NCBI-Hinterlegungsnummer: NM_015015
• UniProt-Hinterlegungsnummer: O94953
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... KDM4B(23030)

Beschreibung

Allgemeine Beschreibung

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), amino acids 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Spezifität

0.32 pmole/min/μg Assay conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM a-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin conjugated secondary antibody followed by Alpha Screening detection.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physikalische Form

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol.

Lagerung und Haltbarkeit

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable