SRP0234

PI3 kinase [p110a(E545K)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥55% (SDS-PAGE)

• Synonym(e): PIK3CA, Phosphoinositide-3-kinase, catalytic, alpha, PtdIns-3-kinase p110
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥55% (SDS-PAGE)
• Form: aqueous solution
• Spezifische Aktivität: ≥800 pmol/min-μg
• Mol-Gew.: 129 kDa; 88 kDa
• Verpackung: pkg of 20 μg
• Konzentration: 0.56 mg/mL
• NCBI-Hinterlegungsnummer: U79143 (p110alpha); XM_043865 (p85alpha)
• UniProt-Hinterlegungsnummer: P42336
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PIK3CA(5290)

Beschreibung

Allgemeine Beschreibung

Complex of N-terminal FLAG-tagged recombinant full-length human p110 (GenBank Accession No. U79143), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Einheitendefinition

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1