SRP0109

HDAC-7 human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(e): HD7, HD7A, HDAC7A, Histone deacetylase 7
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥70% (SDS-PAGE)
• Form: aqueous solution
• Wirksamkeit: ≥10 units/mg
• Mol-Gew.: 78 kDa
• Verpackung: pkg of 2 and 10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: AY302468
• UniProt-Hinterlegungsnummer: Q8WUI4
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... HDAC7(51564)

Beschreibung

Allgemeine Beschreibung

HDAC7 (histone deacetylase 7) is located on human chromosome 12q13. It is expressed in fetal and adult pre-B cells.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem./physiol. Wirkung

Histone deacetylases (HDACs) play crucial roles in the epigenetic modulation of gene expression and helps in the growth, differentiation, and apoptosis of cancer cells. Knockdown of HDAC7 stimulates apoptosis and autophagy in salivary mucoepidermoid carcinoma cells. It may play a vital role in both types of B lymphopoiesis. HDAC7 serves as a repressor of myeloid genes.

Einheitendefinition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable