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SNC50

Sigma-Aldrich

Mission® Small RNA Purification Kit

1 sufficient for 50 preparations

Synonym(e):

microRNA Isolation Kit

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
41105501
NACRES:
NA.52

Qualitätsniveau

Verwendung

sufficient for 50 preparations

Methode(n)

DNA extraction: suitable

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Allgemeine Beschreibung

Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
.

Anwendung

mirPremier® microRNA Isolation Kit has been used to:

  • extract total RNA containing miRNA from grape edible plant derived exosome-like nanoparticles (EPDEN).
  • isolate small RNA from S.sirkka and S. napiecek, and S. arctica.
  • extract miRNA, from frozen rat livers for miRNA analysis.

Leistungsmerkmale und Vorteile

  • Designed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological sources.
  • Enables fast and efficient extraction and concentration of miRNA in 30 minutes for downstream applications.
  • Can extract high-purity miRNA with no detectable large RNA
  • No dangerous organic extractions are involved.

Rechtliche Hinweise

mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany

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Piktogramme

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Signalwort

Warning

Gefahreneinstufungen

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Dongxiao Su et al.
Food & function, 8(2), 808-815 (2017-01-26)
Dietary phenolics exhibit hypolipidemic activity by changing lipid metabolism-related microRNA (miRNA) expression. Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase (quercetin 3-rut-7-rha), rutin and (-)-epicatechin are the main phenolics in lychee (Litchi chinensis Sonn.) pulp. A previous study reported that quercetin 3-rut-7-rha and rutin had hypolipidemic
Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Mu J
Molecular Nutrition And Food Research, 58(7), 1561-1573 (2014)
Jon Bråte et al.
Current biology : CB, 28(20), 3288-3295 (2018-10-16)
The emergence of multicellular animals was associated with an increase in phenotypic complexity and with the acquisition of spatial cell differentiation and embryonic development. Paradoxically, this phenotypic transition was not paralleled by major changes in the underlying developmental toolkit and
Abdulla Abdulla Sabana et al.
Planta, 251(4), 79-79 (2020-03-14)
Genome-wide analysis of small RNAs identifies somatic embryogenesis- specific miRNAs and their targets and provides novel insights into the mechanisms governing somatic embryogenesis in coconut, a highly in vitro recalcitrant species. Coconut, a major plantation crop of the tropics is

Artikel

Die Verfügbarkeit von einfachen Verfahren zum Aufreinigen von DNA und RNA hat die Analyse und Charakterisierung des Genoms und der Genexpression in hohem Maß erleichtert. Es besteht eine Anforderung, DNA und RNA schnell und praktisch aus verschiedenen zellulären Quellen, einschließlich Gewebe aus Tier-, Pflanzen- und Bakterienkulturen, zu isolieren.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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