SAE0003

4-Hydroxyphenylpyruvate Dioxygenase from Streptomyces avermitilis

recombinant, expressed in E. coli

• EC-Nummer: 1.13.11.27 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Form: liquid
• Spezifische Aktivität: ≥5 U/mg
• Mol-Gew.: ~41.8 kDa
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

4-Hydroxyphenylpyruvate Dioxygenase (4-HPPD) is a key enzyme in the catabolism of tyrosine and phenylalanine. 4-HPPD catalyzes the synthesis of homogenistate (2,5-dihydroxyphenylacetate) from ρ-hydroxyphenylpyruvate and molecular oxygen. 4-HPPD also catalyzes the conversion of α-ketoisocaproate to Β-hydroxyisovalerate. 4-HPPD requires Fe²⁺ as an essential cofactor.

Rat 4-HPPD has a minimum of 80% homology with human 4-HPPD. Rat 4-HPPD is expressed abundantly in hepatocytes. It is also expressed in kidney, particularly in epithelial cells of the proximal tubules and the distant tubules. 4-HPPD has been identified as a potential biomarker of drug-induced liver injury in rat studies.

Einheitendefinition

One unit will oxidize 1 μmole of molecular oxygen per minute at pH 7.0, 25 °C, in the presence of 4-hydroxyphenylpyruvate.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable