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Merck

SAB4503501

Sigma-Aldrich

Anti-Cyclin B1 antibody produced in rabbit

affinity isolated antibody

Synonym(e):

CCN-2, CCNB, CCNB1, CCNB1-RS1, CYCB

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Mol-Gew.

antigen 48 kDa

Speziesreaktivität

rat, human, mouse

Konzentration

~1 mg/mL

Methode(n)

ELISA: 1:20000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CCNB1(891)

Allgemeine Beschreibung

Anti-Cyclin B1 Antibody detects endogenous levels of total Cyclin B1 protein.
Cyclin B1 belongs to the cyclin family. It is a 48.337kDa protein. It is present in low level in testis, thymus, bone marrow and smooth muscle. It is mapped to human chromosome 5q13.2.

Immunogen

The antiserum was produced against synthesized peptide derived from human Cyclin B1.

Immunogen Range: 91-140

Anwendung

Anti-Cyclin B1 antibody has been used in western-blot analysis.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem./physiol. Wirkung

Cyclin B1-CDK1 (cyclin-dependent kinase 1) helps to induce chromosome condensation, nuclear lamina resolution and mitotic spindle assembly. It also acts as a potential target for a cancer vaccine.

Leistungsmerkmale und Vorteile

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physikalische Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

CCNB1 (cyclin B1)
Perez-Stable C
Atlas of Genetics and Cytogenetics in Oncology and Haematology (2009)
Identification of a novel Polo-like kinase 1 inhibitor that specifically blocks the functions of Polo-Box domain
Chen Y, et al.
Oncotarget, 8(1), 1234-1246 (2017)
The Tumor Antigen Cyclin B1 Hosts Multiple CD4 T Cell Epitopes Differently Recognized by Pre-Existing Naive and Memory Cells in Both Healthy and Cancer Donors
Chevaleyre C, et al.
Journal of Immunology, 195(4), 1891-1901 (2015)
PIWIL2 promotes progression of non-small cell lung cancer by inducing CDK2 and Cyclin A expression
Qu X, et al.
Journal of Translational Medicine, 13(1), 301-301 (2015)
Yunyu Chen et al.
Oncotarget, 8(1), 1234-1246 (2016-12-03)
Polo-like kinase 1 (Plk1) is a promising target for cancer therapy due to its essential role in cell division. In addition to a highly conserved kinase domain, Plk1 also contains a Polo-Box domain (PBD), which is essential for Plk1's subcellular

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