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SAB4200808

Sigma-Aldrich

Anti-Myosin Light Chain Kinase antibody, Mouse monoclonal

clone K36, purified from hybridoma cell culture

Synonym(e):

Kinase-related protein (KRP), MLCK, Myosin light chain kinase, Smooth muscle, Telokin, smMLCK

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Antikörperform

purified from hybridoma cell culture

Antikörper-Produkttyp

primary antibodies

Klon

K36, monoclonal

Form

buffered aqueous solution

Mol-Gew.

~160 kDa

Speziesreaktivität

bovine, human, mouse, chicken, pig, turkey

Verpackung

antibody small pack of 25 μL

Konzentration

~1 mg/mL

Methode(n)

immunoblotting: 0.25-0.5 μg/mL using chicken gizzard extract

Isotyp

IgG2b

UniProt-Hinterlegungsnummer

Q15746

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Myosin Light Chain Kinase (MLCK) also known as MYLK is a Ca2+/calmodulin dependent myosin light chain phosphorylating agent. This enzyme plays a major role in the phosphorylation of the regulatory light chains of myosin which are essential for the shortening and tension development of smooth muscle cells resulting in smooth muscle contraction. Myosin light chain kinase are widely expressed in many different tissues and cells of eukaryote species. There are two genes mylk1 and mylk2 encoding the MLCK protein, mylk2 is exclusively expressed in skeletal muscle cells.

Immunogen

Purified chicken gizzard myosin light chain kinase.

Anwendung

Monoclonal Anti-Myosin Light Chain Kinase recognizes the myosin light chain kinase of smooth muscle and from non-muscle cells such as cultured fibroblasts. Reactivity has been observed with myosin light chain kinase from chicken, turkey, bovine, human, mouseand pig origin. The antibody is recommended to use in various immunological techniques, including Immunoblotting (~160kDa), Immunohistochemistry and immunofluorescence.

Physikalische Form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Sonstige Hinweise

This product is for R&D use only, not for drug, household, or other uses.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Myosin Light Chain Kinase: A Potential Target for Treatment of Inflammatory Diseases.
Xiong, et al.
Frontiers in Pharmacology, 8, 292-292 (2017)
Renaud Léguillette et al.
American journal of respiratory and critical care medicine, 179(3), 194-204 (2008-11-18)
Airway smooth muscle (SM) of patients with asthma exhibits a greater velocity of shortening (Vmax) than that of normal subjects, and this is thought to contribute to airway hyperresponsiveness. A greater Vmax can result from increased myosin activation. This has
Jeffrey B Wyckoff et al.
Current biology : CB, 16(15), 1515-1523 (2006-08-08)
Tumor cells invading three-dimensional matrices need to remodel the extracellular matrix (ECM) in their path. Many studies have focused on the role of extracellular proteases; however, cells with amoeboid or rounded morphologies are able to invade even when these enzymes
S Topouzis et al.
Developmental biology, 178(2), 430-445 (1996-09-15)
Lineage analysis studies in the avian embryo have identified two types of smooth muscle cells (SMCs) in the tunica media of large elastic arteries; one that originates within the cardiac neural crest and is ectoderm in origin (Ect) and another
Zoe M Goeckeler et al.
The Journal of biological chemistry, 280(38), 33083-33095 (2005-08-02)
This study determined the effects of increased intracellular cAMP and cAMP-dependent protein kinase activation on endothelial cell basal and thrombin-induced isometric tension development. Elevation of cAMP and maximal cAMP-dependent protein kinase activation induced by 10 microm forskolin, 40 microm 3-isobutyl-1-methylxanthine
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