S5816

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse

SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonym(e): SDF-1α/PBSF
• MDL-Nummer: MFCD02100631
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: >97% (SDS-PAGE)
• Form: powder
• Wirksamkeit: 0.15-0.6 ng/mL ED₅₀
• Qualität: endotoxin tested
• Mol-Gew.: predicted mol wt ~8 kDa
• Verpackung: pkg of 10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <1 EU/μgtested (LAL test)
• UniProt-Hinterlegungsnummer: P40224
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Cxcl12(20315)

Beschreibung

Allgemeine Beschreibung

Stromal derived factor-1 (SDF-1) is localized to adipocytes.

Anwendung

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.

Biochem./physiol. Wirkung

SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..

Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Angaben zur Herstellung

Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.

Hinweis zur Analyse

The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)