S0698

Anti-SmB antibody, Mouse Monoclonal

clone 12F5, purified from hybridoma cell culture

• Synonym(e): Anti-SNRPB1, Anti-Sm protein B, Anti-Sm-B, Anti-SmB/SmB, Anti-Small nuclear ribonucleoprotein associated protein B, Anti-snRNP-B
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: 12F5, monoclonal
• Mol-Gew.: antigen ~25 kDa
• Speziesreaktivität: human, mouse, canine
• Methode(n): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.5-1 μg/mL using HeLa nuclear cell extract
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P14678
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SNRPB(6628) ; mouse ... Snrpb(20638)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-SmB (mouse IgG1 isotype) is derived from the hybridoma 12F5-H3.D8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an SmB recombinant protein.

SNRPB (small nuclear ribonucleoprotein-associated protein B) is an important constituent of the spliceosome. It codes for SmB/B′ (small nuclear ribonucleoprotein polypeptides B and B1). It is located on human chromosome 20p13. SmB′/B are upregulated in all tissues excluding postnatal brain.

Immunogen

SmB recombinant protein.

Anwendung

• Monoclonal Anti-SmB antibody produced in mouse has been used in:
• western blotting
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• immunocytochemistry

Biochem./physiol. Wirkung

SNRPB (small nuclear ribonucleoprotein-associated protein B) mutations results in cerebro-costo-mandibular syndrome. Downregulation of SNRPB affects the upregulation and processing of RNA splicing machinery components. It also plays a major role in RNA stability.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)