S0191

Sonic Hedgehog Peptide from mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, Fused to a histidine tag at the C-terminus, lyophilized powder

• Synonym(e): Shh
• MDL-Nummer: MFCD03792594
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 0.4-3.0 μg/mL ED₅₀
• Mol-Gew.: predicted mol wt 20 kDa
• Verpackung: pkg of 25 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Verunreinigungen: <1 EU/μg
• UniProt-Hinterlegungsnummer: Q62226
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Shh(20423)

Beschreibung

Allgemeine Beschreibung

Sonic hedgehog (Shh) is a morphogenic glycoprotein. The gene encoding it is localized on mouse chromosome 5.

Anwendung

Sonic hedgehog peptide from mouse has been used for neural differentiation.

Biochem./physiol. Wirkung

Cell signaling peptide involved in embryonic morphogenic development of several tissues including Hensen′s node, the notochord, and the neural tube.

Sonic hedgehog (Shh) has a role in fracture healing and regeneration. The protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer.

Sonstige Hinweise

N-terminal peptide encoding amino acid residues 25-198.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4, containing 5% trehalose and 50 μg of bovine serum albumin per 1 μg of cytokine.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable