R6265

EcoR I from Escherichia coli BS5

Restriction Enzyme

• CAS-Nummer: 80498-17-5
• EC-Nummer: 3.1.21.4 (BRENDA, IUBMB)
• EG-Nummer: 279-487-9
• MDL-Nummer: MFCD00163532
• UNSPSC-Code: 12352204
• NACRES: NA.53

Eigenschaften

• Qualität: for molecular biology
• Form: buffered aqueous glycerol solution
• Konzentration: 10,000 units/mL
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Spezifität

Recognition sequence: 5′-G/AATTC-3′
Cutting results: a 2-10-fold EcoR I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 20 minutes.

Anwendung

EcoRI is a restriction endonuclease that is used in molecular biology applications to cleave DNA at the recognition site 5′-G/AATTC-3′, generating fragments with 5′-cohesive termini.

Sonstige Hinweise

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Comment: Avoid suboptimal reaction conditions such as low salt, high pH (>8.0) and high glycerol (>5%) as they will alter EcoRI specificity and precipitate star activity.

Einheitendefinition

One unit is the enzyme activity that completely cleaves 1 mg of λDNA in 1 hour at 37 °C in a total volume of 50 ml of 1x digestion buffer SH for restriction enzymes. 1 mg pBR322 DNA is digested completely by 2 units of EcoR I.

Physikalische Form

Solution in 10 mM Tris-HCl, pH 7.2 , 1 mM EDTA, 200 mM NaCl , 0.5 mM dithioerythritol, 50% glycerol (v/v), 0.2% Triton X- 100 (v/v), 4°C

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)