R5404

Monoclonal Anti-Rab9 antibody produced in mouse

~2 mg/mL, clone Rab9-29, purified immunoglobulin, buffered aqueous solution

• MDL-Nummer: MFCD03097193
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: Rab9-29, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: ~23 kDa
• Speziesreaktivität: human, rat, hamster, canine, mouse
• Konzentration: ~2 mg/mL
• Methode(n): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 2 μg/mL using total cell extract of HeLa cells.
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P51151
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RAB9A(9367) ; mouse ... Rab9(56382) ; rat ... Rab9a(84589)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-Rab9 (mouse IgG1 isotype) is derived from the hybridoma Rab9-29 produced by the fusion of mouse cells (NS1 cells) and splenocytes from BALB/c mice. Ras-related protein Rab-9A (Rab9) is a member of the Rab family of small guanosine triphosphatases (GTPases). The gene Rab9 is mapped to human chromosome Xp22.2. Rab9 GTPase is localized predominantly to late endosomes.

Spezifität

Monoclonal Anti-Rab9 recognizes human, canine, rat, mouse and hamster Rab9 (approx. 23 kDa)

Immunogen

synthetic peptide corresponding to amino acids 187-200 of human Rab9, conjugated to KLH.

Anwendung

Monoclonal Anti-Rab9 antibody produced in mouse has been used in:

• western blotting
• enzyme-linked immunosorbent assay(ELISA)
• immunoprecipitation
• immunocytochemistry

Biochem./physiol. Wirkung

Ras-related protein Rab-9A (Rab9) guanosine triphosphatase (GTPase) is required for the transport of mannose 6-phosphate receptors (MRPs) from endosomes to the trans-Golgi network (TGN). Rab GTPases are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Rab9 GTPase in its GTP bound conformation binds specifically to an effector protein named p40 that stimulates the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the TGN. Rab9 also interacts with the vesicle cargo selection protein tail-interacting protein of 47 kDa (TIP47). This interaction is required for Rab9 stability on late endosomes and may be crucial for vesicular transport in the virus life cycle.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)