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Merck

R0886

Sigma-Aldrich

Monoclonal Anti-Rat IgM antibody produced in mouse

clone RTM-32, ascites fluid

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

mouse

Konjugat

unconjugated

Antikörperform

ascites fluid

Antikörper-Produkttyp

secondary antibodies

Klon

RTM-32, monoclonal

Enthält

15 mM sodium azide

Methode(n)

capture ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: 1:1,000
western blot: suitable

Isotyp

IgG1

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Monoclonal anti-Rat IgM (mouse IgG1 isotype) is derived from the RTM-32 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a rat IgM myeloma preparation. Immunoglobulin M (IgM) is the first immunoglobulin to be produced, which is detected as early as 9 weeks of gestation. It has a pentameric structure, in which monomers are linked together by disulfide bonds. IgM is produced by B cells and is expressed in high concentrations in blood.
Rat immunoglobulins have seven distinct classes, namely, IgM, IgA, IgE, IgG1, IgG2a, IgG2b and IgG2c. Rat IgMs have been implicated in complement activation . Rat IgMs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgM is a useful tool for the analysis of target proteins
Monoclonal Anti-Rat IgM antibody is specific for an epitope present on the heavy chain of rat IgM. By immunoblotting, the antibody detects the denatured-reduced heavy chain molecule of rat IgM (μ-chain). The product recognizes rat IgMs derived from normal serum or myeloma proteins, but does not detect other rat immunoglobulins. In assays such as indirect ELISA and dot blot, the antibody weakly associates with guinea pig immunoglobulins. The product does not bind to IgG or serum preparations obtained from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.

Spezifität

Monoclonal anti-Rat IgM recognizes an epitope located on the heavy chain of rat IgM. The antibody detects the rat IgM derived from normal serum or myeloma proteins, but not the other rat immunoglobulins.

Immunogen

Rat IgM myeloma preparation

Anwendung

Monoclonal Anti-Rat IgM antibody is suitable for use in:
  • indirect ELISA (1:1,000)
  • immunocytochemistry
  • immunohistochemistry
  • dot blot
  • immunoblotting

Biochem./physiol. Wirkung

Immunoglobulin (IgM) serves as the first line of defense during microbial infections. It serves as the antigen receptor of naive B cells. Secreted IgM (sIgM) is implicated in B cell maturation and complement activation. Detection of IgM antibodies in a neonate′s serum may indicate intrauterine infection (e.g. congenital rubella syndrome). Development of anti-donor IgM after combined liver-kidney transplantation provides a graft-protecting effect. Anti-Human IgM (μ-specific) antibodies are used in the detection of IgM-associated pathologies, such as selective IgM immunodeficiency (SIgMID) and the hyper immunoglobulin M (hyper-IgM or HIGM) syndromes.

Physikalische Form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Niubel Diaz Padilla et al.
Surgery, 142(5), 722-733 (2007-11-06)
The role of C-reactive protein (CRP), natural immunoglobulin M (IgM), and natural IgM against phosphorylcholine (anti-Pc IgM) was investigated in relation with complement activation in a rat model of intestinal ischemia and reperfusion (II/R). The effect of C1-esterase inhibitor (C1-Inh)
Rachael Racine et al.
Immunology letters, 125(2), 79-85 (2009-06-23)
Much has been learned about the structure, function, and production of IgM, since the antibody's initial characterization. It is widely accepted that IgM provides a first line of defense during microbial infections, prior to the generation of adaptive, high-affinity IgG
Trang T T Nguyen et al.
Critical reviews in immunology, 36(2), 163-177 (2016-12-03)
Most serum immunoglobulin M (IgM) is "natural IgM", which is produced apparently spontaneously by a distinct subset of B cells requiring no exogenous antigenic or microbial stimuli. Natural IgM is an evolutionarily conserved molecule and reacts with a variety of
Graham A Tipples et al.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 30(3), 233-238 (2004-05-12)
Rubella virus generally causes a mild fever, rash illness similar in clinical presentation to infections by other viruses including measles and parvovirus B19. Rubella infections in pregnant women in the first trimester carry a high risk of congenital rubella syndrome
Mohan S Maddur et al.
Clinical reviews in allergy & immunology, 58(2), 213-228 (2019-06-05)
Natural antibodies (nAbs) are most commonly defined as immunoglobulins present in the absence of pathological conditions or deliberate immunizations. Occurrence of nAbs in germ- and antigen-free mice suggest that their production is driven, at least in part, by self-antigens. Accordingly

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