P8913

Phospholipase A₂ aus Rinderpankreas

lyophilized powder, ≥20 units/mg protein

• Synonym(e): Lecithinase A, PLA₂
• CAS-Nummer: 9001-84-7
• EC-Nummer: 3.1.1.4 (BRENDA, IUBMB)
• EG-Nummer: 232-637-7
• MDL-Nummer: MFCD00131890
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Form: lyophilized powder
• Spezifische Aktivität: ≥20 units/mg protein
• Zusammensetzung: Protein, 5-20% biuret
• UniProt-Hinterlegungsnummer: P00593
• Lagertemp.: −20°C
• Angaben zum Gen: cow ... PLA2G1B(282457)

Beschreibung

Allgemeine Beschreibung

Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme.

Anwendung

Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels.

Biochem./physiol. Wirkung

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Verpackung

Package size based on protein content

Einheitendefinition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C.

Physikalische Form

Contains Tris buffer salts.

Hinweis zur Analyse

Protein determined by biuret.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)