P0493

Monoclonal Anti-PRMT5 antibody produced in mouse

~2 mg/mL, clone PRMT5-21, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-Protein-Arginine Methyl Transferase 5
• MDL-Nummer: MFCD03792429
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: PRMT5-21, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~70 kDa by SDS-PAGE
• Speziesreaktivität: mouse, hamster, bovine, canine, monkey, rat, human, chicken
• Konzentration: ~2 mg/mL
• Methode(n): indirect ELISA: suitable; microarray: suitable; western blot: 2 μg/mL using whole cell extract of human melanoma cell line G361
• Isotyp: IgG2a
• UniProt-Hinterlegungsnummer: O14744
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PRMT5(10419)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-PRMT5 (mouse IgG2a isotype) is derived from the PRMT5-21 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with GST-PRMT5 containing the C-terminal region of the human PRMT5. Protein arginine methyltransferases (PRMT) 5 belongs to the type II arginine methyltransferases. PRMT5 (also known as Skb1Hs/janus kinase-binding protein 1 (JBP1) exists as homo-oligomeric complexes, which includes a dimer and tetramer. PRMT5 is found mainly in the cytoplasm. This gene is located on human chromosome 14q11.2.

Immunogen

GST-PRMT5 containing the C-terminal region of the human PRMT5 (amino acids 315-637).

Anwendung

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-PRMT5 antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem./physiol. Wirkung

Protein arginine methyltransferase 5 (PRMT5) participates in the symmetrical dimethylation of arginine residues. Homooligomerization of PRMT5 has an important role in its ability to methylate the myelin basic protein (MBP) protein. PRMT5 also binds the Janus kinases and are involved in an interferon-signaling pathway. It also helps to produce symmetrical ω-NG-dimethylarginine.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable