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Merck

P0117

Sigma-Aldrich

Monoklonales Anti-PIAS3 in Maus hergestellte Antikörper

~2 mg/mL, clone PIA3, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Anti-Protein Inhibitor of Activated STAT 3

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

PIA3, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~68 kDa (additional band at 44 kDa may be present)

Speziesreaktivität

chicken, mouse, canine, human, bovine

Konzentration

~2 mg/mL

Methode(n)

immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 2-4 μg/mL using HepG2 total cell extract

Isotyp

IgG1

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... PIAS3(10401)
mouse ... Pias3(229615)

Allgemeine Beschreibung

Protein inhibitor of activated STAT protein 3 (PIAS3) is an inhibitor of activated STAT3. The gene encodes for a 619-amino acid protein and has a molecular mass of 68 kDa. PIAS3 shares 55% identity with PIAS1 and PIASX proteins and 39% identity with PIASY. PIAS3 is widely expressed in various human tissues.

Immunogen

synthetic peptide corresponding to amino acids 591-604 of human PIAS3.

Anwendung

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-PIAS3 antibody produced in mouse has been used in:
  • enzyme linked immuno sorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry

Biochem./physiol. Wirkung

Protein inhibitor of activated STAT protein 3 (PIAS3) and other members of this family can act as small ubiquitin-like modifier (SUMO) E3 ligases and interfere with the transcription activity of many transcription factors. PIAS3 binds to microphthalmia-associated transcription factor (MITF), a key DNA-binding protein. It blocks MITF DNA-binding activity and co-expression of MITF. PIAS3 in NIH 3T3 cells inhibits MITF-driven transcription activity.

Zielbeschreibung

PIAS3 encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

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Analysenzertifikate (COA)

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Jennifer C Miller et al.
Nature immunology, 13(9), 888-899 (2012-07-17)
Although much progress has been made in the understanding of the ontogeny and function of dendritic cells (DCs), the transcriptional regulation of the lineage commitment and functional specialization of DCs in vivo remains poorly understood. We made a comprehensive comparative
PIAS proteins and transcriptional regulation?more than just SUMO E3 ligases?
Sharrocks AD
Genes & Development, 20(7), 754-758 (2006)
PIAS3 may represent a potential biomarker for diagnosis and therapeutic of human colorectal cancer
Li H, et al.
Medical Hypotheses, 81(6), 1151-1154 (2013)
A new role for the STAT3 inhibitor, PIAS3 a repressor of microphthalmia transcription factor
Levy C, et al.
The Journal of Biological Chemistry, 277(3), 1962-1966 (2002)
Zohar Yagil et al.
Journal of immunology (Baltimore, Md. : 1950), 182(4), 2168-2175 (2009-02-10)
Protein inhibitor of activated STAT3 (PIAS3) functions in vivo as a key molecule in suppressing the transcriptional activity of both microphthalmia transcription factor (MITF) and STAT3, two transcription factors that play a major role in the development, phenotypic expression, and

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