P0073

Anti-PERK (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Anti-E2AK3, Anti-EIF2AK3, Anti-HsPEK, Anti-PEK, Anti-PRKR-like endoplasmic reticulum kinase, Anti-Pancreatic eIF2α kinase, Anti-WRS
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Speziesreaktivität: mouse, rat
• Methode(n): western blot: 1:500-1:1,000 using whole cell lysates of HEK-293T expressing PERK
• UniProt-Hinterlegungsnummer: Q9Z1Z1
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Eif2ak3(13666) ; rat ... Eif2ak3(29702)

Beschreibung

Allgemeine Beschreibung

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is one of the three mammalian UPR (unfolded protein response) transducers; the other two being activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 (IRE1). It is a highly conserved eIF2a (eukaryotic initiation factor 2 α) kinase.

Spezifität

Anti-PERK (N-terminal) specifically recognizes mouse and rat PERK.

Immunogen

synthetic peptide corresponding to amino acids 124-139 of mouse PERK, conjugated to KLH. This sequence is identical in rat and mouse.

Anwendung

Anti-PERK (N-terminal) antibody produced in rabbit may be used in immunoblotting.

Biochem./physiol. Wirkung

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an ER stress sensor which phosphorylates eukaryotic initiation factor 2 α (eIF2α), thereby reducing the translation of most messenger RNAs (mRNAs). This results in reduction of protein load in the ER lumen. In S63del-Charcot-Marie-Tooth 1B neuropathy mice model, the suppression of PERK results in improved myelination. In mice models of frontotemporal dementia, the suppression of translation in a PERK-eIF2α-dependent manner plays a key role in neuronal loss. Administration of GSK2606414, a PERK inhibitor, results in suppression of tau-mediated neurodegeneration. PERK confers protection to heart against pressure overload-induced congestive heart failure. Mutations in this gene are linked with Wolcott-Rallison syndrome (WRS) in humans, which is characterized by permanent neonatal diabetes, exocrine-deficient pancreas, growth retardation, and osteopenia.

Zielbeschreibung

PERK (also known as eIF2AK3, PEK and WRS) is a transmembrane kinase that is highly expressed in thepancreas. It resides in the ER and couples stress signals initiated by protein malfolding in the ER lumen to eIF2α phosphorylation and reduces protei

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)