N9914

Polynucleotide phosphorylase from Synechocystis sp.

recombinant, expressed in E. coli

• Synonym(e): PNPase, Polyribonucleotide Nucleotidyltransferase
• EC-Nummer: 2.7.7.8 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131992
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: bacterial (Synechocystis sp.)
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Beschreibung: Histidine tagged
• Assay: 90% (SDS-PAGE)
• Form: solution
• Spezifische Aktivität: ≥500 units/mg protein
• Mol-Gew.: 85 kDa
• Methode(n): cell based assay: suitable
• Eignung: suitable for molecular biology
• Anwendung(en): cell analysis
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase.

Anwendung

Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor.

Biochem./physiol. Wirkung

Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity.

Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells.

Einheitendefinition

One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C.

Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl₂, 60 mM KCl, 20% (w/v) Glycerol

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable