N7162

Anti-Neuroglobin

~1.2 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Ngb
• MDL-Nummer: MFCD09265363
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Speziesreaktivität: rat, human, mouse
• Konzentration: ~1.2 mg/mL
• Methode(n): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5.0 μg/mL using biotin/ExtrAvidin^®-Peroxidase staining of heat-retrieved rat brain sections; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5.0-10.0 μg/mL using biotin/ExtrAvidin^®-Peroxidase staining of heat-retrieved human and mouse brain sections
• UniProt-Hinterlegungsnummer: Q9NPG2
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NGB(58157)

Beschreibung

Allgemeine Beschreibung

Neuroglobin is an oxygen-binding heme protein predominantly expressed in the nervous system in man and other vertebrates. Neuroglobin belongs to the globin superfamily. It displays the typical globin fold, but it shares little amino acid sequence similarity with vertebrate hemoglobins and myoglobins. Neuroglobin is highly conserved among vertebrates and is evolutionary related to the invertebrate nerve globins. In mouse and rat, neuroglobin is preferentially expressed in neuronal cells of the central and peripheral nervous systems, but also in some endocrine tissues. Neuroglobin is highly expressed in neurons of mammalian retina.

Immunogen

synthetic peptide corresponding to amino acids 55-70 of human neuroglobin, conjugated to KLH.

Anwendung

Anti-Neuroglobin antibody produced in rabbit has been used in

• immunofluorescence double staining
• immunostaining
• immunohistochemistry

Biochem./physiol. Wirkung

Neuroglobin may play an essential role in oxygen homeostasis of neuronal cells. Neuroglobin is thought to protect neurons from hypoxic-ischemic injury as suggested by its increased expression under hypoxic conditions in- vitro as well as in focal cerebral ischemia in vivo. Neuronal survival after hypoxia decreased when neuroglobin expression is inhibited by antisense oligonucleotide and enhanced by neuroglobin overexpression. Its distribution correlates with the subcellular localization of mitochondria and with oxygen demands, possibly linking neuroglobin to cellular respiration.

Zielbeschreibung

Neuroglobin is an oxygen-binding heme proteinpredominantly expressed in the nervous system.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Rechtliche Hinweise

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)