MAK479

NADP/NADPH Assay Kit

sufficient for 100 colorimetric tests

• UNSPSC-Code: 12161503
• NACRES: NA.84

Eigenschaften

• Qualitätsniveau: 100
• Nachweisverfahren: colorimetric
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Leistungsmerkmale und Vorteile

• The linear detection range of the kit is 0.1 µM to 10 µM NADP+ /NADPH
• Compatible with high-throughput handling systems.
• Convenient, the procedure involves adding a single working reagent, and reading the optical density at time zero and 30 minutes at room temperature.

Eignung

The kit is suitable for detection of NADP+ /NADPH concentrations and ratios in cell or tissue extracts.

Prinzip

The NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ /NADPH and is not interfered by NAD+ /NADH.

Sicherheitshinweise

• Piktogramme: GHS02,GHS05,GHS07
• Signalwort: Danger
• H-Sätze: H225,H290,H315,H319,H336
• P-Sätze: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 3 - Flammable liquids
• WGK: WGK 2