MAK226
Enrofloxacin Extraction Kit
Sufficient for 40 extractions
About This Item
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Grünere Alternativprodukt-Eigenschaften
Waste Prevention
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sustainability
Greener Alternative Product
Anwendung(en)
cosmetics
food and beverages
sample preservation
Grünere Alternativprodukt-Kategorie
Lagertemp.
room temp
Allgemeine Beschreibung
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.
Anwendung
Click on the following link for a video demonstration of how the kit works
Leistungsmerkmale und Vorteile
Eignung
Prinzip
Enrofloxacin is typically extracted using a single solvent system containing 80% methanol in buffer. In the standard procedure, methanol containing the enrofloxacin is separated from tissue by centrifugation for 10 minutes followed by manual pipetting. The extract is then dried and reconstituted into 1 mL of 8% methanol in sample dilution buffer. The extract is subsequently defatted by adding 1 mL hexane and centrifuging. A 50 μL aliquot is taken from the bottom layer containing the defatted enrofloxacin extract and used for analysis by ELISA and spectrophotometry.
The Enrofloxacin Extraction Kit shortcuts the extraction process by eliminating the need for centrifugation, manual pipetting, and post-extraction defatting with hexane or another lipophilic solvent. After adding 80% methanol to the sample, it can be mixed manually for 15 minutes or with sonication for 5 minutes and poured through the syringe containing a filter. The eluted solution containing the enrofloxacin extract is then reconstituted in 8% methanol and a 50 μL aliquot is used for analysis by ELISA and spectrophotometry.
Vergleichbares Produkt
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Zielorgane
Eyes
Lagerklassenschlüssel
3 - Flammable liquids
Flammpunkt (°F)
49.5 °F
Flammpunkt (°C)
9.7 °C
Analysenzertifikate (COA)
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