M7279

N,N′-Methylenbisacrylamid

powder, for molecular biology, suitable for electrophoresis, ≥99.5%

• Synonym(e): BIS
• Lineare Formel: (CH₂=CHCONH)₂CH₂
• CAS-Nummer: 110-26-9
• Molekulargewicht: 154.17
• Beilstein: 1706297
• EG-Nummer: 203-750-9
• MDL-Nummer: MFCD00008625
• UNSPSC-Code: 41105319
• PubChem Substanz-ID: 24897151
• NACRES: NB.22

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Assay: ≥99.5%
• Form: powder
• Methode(n): electrophoresis: suitable
• Verunreinigungen: ≤0.05% Free carboxylic acid (T); ≤50 mg/kg Total sulfur (as SO₄ ) (ICP)
• Leitfähigkeit: ≤10 μmho (2% solution)
• Anionenspuren: chloride (Cl^-): ≤50 mg/kg
• Kationenspuren: Ca: ≤10 ppm; Cd: ≤5 ppm; Co: ≤5 ppm; Cr: ≤5 ppm; Cu: ≤5 ppm; Fe: ≤5 ppm; K: ≤50 ppm; Mg: ≤5 ppm; Mn: ≤5 ppm; Na: ≤50 ppm; Ni: ≤5 ppm; Pb: ≤5 ppm; Zn: ≤5 ppm
• Absorption: ≤0.2 at 290 at 1%
• Fremdaktivität: DNase and RNase, none detected
• SMILES String: C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)
• InChIKey: ZIUHHBKFKCYYJD-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Anwendung

N,N′-Methylenebisacrylamide has been used:

• as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix
• as a crosslinker to prepare tough hydrogel network
• in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

Zum Mischen mit Acrylamid für die Herstellung von Polyacrylamidgelen für den Einsatz in Protein- und Nukleinsäure-Elektrophorese.

Sicherheitshinweise

• Piktogramme: GHS06,GHS08
• Signalwort: Danger
• H-Sätze: H301,H312,H340,H350,H361fd,H372
• P-Sätze: P202 - P260 - P264 - P280 - P301 + P310 - P302 + P352 + P312
• Gefahreneinstufungen: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral
• Zielorgane: Peripheral nervous system
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves