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M5257

Sigma-Aldrich

Apfelsäure-Dehydrogenase (Oxalacetat-decarboxylierend) aus Hühnerleber

ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)

Synonym(e):

‘Malic Enzyme’

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About This Item

CAS-Nummer:
9028-47-1
EC-Nummer:
1.1.1.40 (BRENDA, IUBMB)
EG-Nummer:
232-834-8
MDL-Nummer:
MFCD00131568
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

chicken liver

Form

ammonium sulfate suspension

Spezifische Aktivität

10-30 units/mg protein (modified Warburg-Christian)

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.

Anwendung

Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.

Biochem./physiol. Wirkung

Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.

Einheitendefinition

One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25 °C.

Physikalische Form

Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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A R Ashton
Archives of biochemistry and biophysics, 345(2), 251-258 (1997-10-06)
NADP-malic enzyme (NADP-ME, EC 1.1.1.40) was purified to near-homogeneity from leaves of the C4 dicot Flaveria bidentis and shown to possess intrinsic NAD-dependent malic enzyme activity. The NAD-dependent activity is optimal at pH 7.5 and in the presence of Mn2+.
Synthesis and Degradation of Malic Enzyme in Chick Liver.
Silpananta, P
The Journal of Biological Chemistry, 264, 5754-5761 (1971)
K N Rao et al.
Gastroenterology, 113(1), 238-248 (1997-07-01)
We showed previously that the peroxisome proliferators di(2-ethylhexyl)phthalate (DEHP), clofibrate, and 4-chloro-6-(2,3 xylidino)-2-pyrimidinylthio (N-beta-hydroxyl)acetamide (BR931) alter hepatic sex steroid metabolism and receptor expression during induction of hepatic hyperplasia and hepatocellular carcinoma (HCC) in rats. The aim of this study was
Establishment and biological characterization of fibroblast cell line from the Langshan chicken
Guan, W., et al.
Cell Proliferation, 42, 157-163 (2010)
Dietary manganese requirement of juvenile yellow catfish Pelteobagrus fulvidraco, and effects on whole body mineral composition and hepatic intermediary metabolism
Tan, X., et al.
Aquaculture (Amsterdam, Netherlands), 326, 68-73 (2012)
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Artikel

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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