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Merck

M1018

Sigma-Aldrich

Minimum Essential Medium Eagle

With Hanks′ salts, ʟ-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

MEM

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

product name

Minimum Essential Medium Eagle, With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Menge

Formulated to contain 10.7 grams of powder per liter of medium.

Rekonstituierung

Supplement with 0.35 g/L sodium bicarbonate.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protokolle

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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