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M0269

Sigma-Aldrich

Methotrexat−Agarose

saline suspension

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About This Item

MDL-Nummer:
MFCD00164466
UNSPSC-Code:
13111023
NACRES:
NA.56

Biologische Quelle

plant

Qualitätsniveau

200

Form

saline suspension

Kennzeichnungsgrad

2-7 mg per mL

Methode(n)

affinity chromatography: suitable

Matrix

cross-linked 4% beaded agarose

Matrixaktivierung

cyanogen bromide

Matrixanbindung

carboxyl

Matrix-Spacer

8 atoms

Eignung

suitable for chromatography

Lagertemp.

2-8°C

Anwendung

Methotrexate-agarose is used in protein chromatography, affinity chromatography, metabolic pathways and specialty resins. Methotrexate-agarose has been used to investigate the toxicity mechanism of mortality in adult buffalo flies caused by ingestion of folate analogues. Methotrexate-agarose has also been used to study the purification, cloning, and functional expression of dihydroneopterin triphosphate 2′-epimerase from Escherichia coli.

Physikalische Form

Suspension in 1.0 M NaCl, enthält 0.02% Thiomersal

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Analysenzertifikate (COA)

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H J Chung et al.
Biochimica et biophysica acta, 1524(2-3), 183-188 (2000-12-13)
Tetrahydrobiopterin (BH4)-glucoside was identified from Synechococcus sp. PCC 7942 by HPLC analysis and the enzymatic activity of a glycosyltransferase producing the compound from UDP-glucose and BH4. The novel enzyme, named UDP-glucose:BH4 glucosyltransferase, has been purified 846-fold from the cytosolic fraction
M Iwakura et al.
Protein engineering, 5(8), 791-796 (1992-12-01)
A single polypeptide chain containing two dihydrofolate reductase (DHFR) sequences from Escherichia coli was constructed to determine if a repeat sequence fusion protein could be expressed in an active form. The possibility that intersequence interactions could play a significant role
Ying Xu et al.
Journal of bacteriology, 185(18), 5519-5526 (2003-09-02)
Adapting metabolic enzymes of microorganisms to low temperature environments may require a difficult compromise between velocity and affinity. We have investigated catalytic efficiency in a key metabolic enzyme (dihydrofolate reductase) of Moritella profunda sp. nov., a strictly psychrophilic bacterium with
Gottfried Wilharm et al.
BMC microbiology, 4, 27-27 (2004-07-14)
Pathogenic Yersinia species (Y. enterocolitica, Y. pestis, Y. pseudotuberculosis) share a type three secretion system (TTSS) which allows translocation of effector proteins (called Yops) into host cells. It is believed that proteins are delivered through a hollow needle with an
Kazumi Urakawa et al.
European journal of pharmacology, 435(2-3), 237-244 (2002-02-01)
N-[[4-[(2,4-diaminopteridin-6-yl)methyl]-3,4-dihydro-2H-1,4-benzothiazin-7-yl]-carbonyl]-L-homoglutamic acid (MX-68), a derivative of methotrexate, was chemically designed to resist polyglutamation and to have a high affinity for dihydrofolate reductase, in an attempt to reduce the side effects of methotrexate. We confirmed that MX-68 did not undergo polyglutamation
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