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L5024

Sigma-Aldrich

Lipopolysaccharide aus Escherichia coli O127:B8

purified by ion-exchange chromatography, TLR ligand tested

Synonym(e):

LPS

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About This Item

EG-Nummer:
297-473-0
MDL-Nummer:
MFCD00212998
UNSPSC-Code:
12352201
NACRES:
NA.25

Biologische Quelle

Escherichia coli (O127:B8)

Qualitätsniveau

200

Form

lyophilized powder

Aufgereinigt durch

ion-exchange chromatography

Verunreinigungen

<1% Protein
<1% RNA

Farbe

white to yellow cast

Löslichkeit

water: soluble

Versandbedingung

ambient

Lagertemp.

2-8°C

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Verwandte Kategorien

Allgemeine Beschreibung

This product is extracted from E. coli serotype O127:B8 and purified by ion exchange. The source strain is ATCC 12740. This LPS serotype has been used in the study of septic shock and to induce NOS in murine macrophages and PAF synthesis in rat glomerular masangial cells.

Anwendung

Lipopolysaccharide (LPS) sind charakteristische Bestandteile der Zellwand von gramnegativen Bakterien. LPS und seine Lipid-A-Gruppe stimulieren Zellen des angeborenen Immunsystems durch den Toll-like-Rezeptor 4 (TLR4). TLR4 gehört zur Toll-like-Rezeptor-Proteinfamilie, die häufige Pathogen-assoziierte molekulare Strukturen (PAMPs) erkennt.

Biochem./physiol. Wirkung

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Angaben zur Herstellung

The product is soluble in water (5 mg/mL) or cell culture medium (1 mg/mL) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/mL) has been achieved in aqueous saline after vortexing and warming to 70-80 °C. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sonstige Hinweise

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Beschreibung
Preisangaben

Piktogramme

Skull and crossbones
GHS06

Signalwort

Danger

H-Sätze

H300

Gefahreneinstufungen

Acute Tox. 2 Oral

Lagerklassenschlüssel

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Mingju Cao et al.
Frontiers in cellular neuroscience, 9, 294-294 (2015-08-25)
Neuroinflammation in utero may result in life-long neurological disabilities. The molecular mechanisms whereby microglia contribute to this response remain incompletely understood. Lipopolysaccharide (LPS) or saline were administered intravenously to non-anesthetized chronically instrumented near-term fetal sheep to model fetal inflammation in
M Cortes et al.
Scientific reports, 7(1), 10645-10645 (2017-09-08)
Neuroinflammation in utero may result in life-long neurological disabilities. Microglia play a pivotal role, but the mechanisms are poorly understood. No early postnatal treatment strategies exist to enhance neuroprotective potential of microglia. We hypothesized that agonism on α7 nicotinic acetylcholine
Kristen R Hollinger et al.
Journal of Alzheimer's disease : JAD, 77(1), 437-447 (2020-07-18)
Given the emergent aging population, the identification of effective treatments for Alzheimer's disease (AD) is critical. We investigated the therapeutic efficacy of JHU-083, a brain-penetrable glutamine antagonist, in treating AD using the humanized APOE4 knock-in mouse model. Cell culture studies
Manman Geng et al.
Clinical immunology (Orlando, Fla.), 220, 108579-108579 (2020-09-01)
Endoplasmic reticulum (ER) stress associated proteins contribute to the pathogenesis of rheumatoid arthritis (RA) through affecting synoviocyte proliferation and proinflammatory cytokine production. The role of DERL3, an ER-associated degradation component, in joint inflammation of RA was explored. Synovial tissues from
Chen Seng Ng et al.
Cell death and differentiation, 27(8), 2363-2382 (2020-02-09)
Host nucleases are implicated in antiviral response through the processing of pathogen-derived nucleic acids. Among many host RNases, decapping enzymes DCP1 and 2, and 5'→3' exonuclease XRN1, which are components of the RNA decay machinery, have been extensively studied in
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Verwandter Inhalt

Lipopolysaccharides

Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is localized in the outer layer of the membrane and is, in noncapsulated strains, exposed on the cell surface.

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