L2012

Lipopolysaccharide aus Salmonella enteritidis

purified by gel-filtration chromatography

• Synonym(e): LPS
• MDL-Nummer: MFCD00131520
• UNSPSC-Code: 12352201
• NACRES: NA.25

Eigenschaften

• Biologische Quelle: Salmonella enterica (Serotype enteritidis)
• Qualitätsniveau: 200
• Form: lyophilized powder
• Aufgereinigt durch: gel-filtration chromatography
• Verunreinigungen: <1% Protein
• Farbe: white to faint yellow
• Löslichkeit: water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

This product is extracted from Salmonella enteritidis and purified by gel filtration. The source strain is ATCC 13076. Lipopolysaccharide from Salmonella enteritidis is mutagenic in murine spleen cells and causes a plaque-forming response in those cells.

Anwendung

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4).

Biochem./physiol. Wirkung

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Angaben zur Herstellung

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sonstige Hinweise

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable