Direkt zum Inhalt
Merck
Alle Fotos(1)

Key Documents

View All Documentation

I8523

Sigma-Aldrich

Interleukin-12 from mouse

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(e):

IL-12

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

MDL-Nummer:
MFCD00212975
UNSPSC-Code:
12352202
NACRES:
NA.32

Biologische Quelle

mouse

Qualitätsniveau

100

Rekombinant

expressed in baculovirus infected Sf21 cells

Assay

≥97% (SDS-PAGE)

Form

lyophilized powder

Wirksamkeit

0.01-.1 ng/mL ED50

Mol-Gew.

glycoprotein 70 kDa

Verpackung

pkg of 10 μg

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

UniProt-Hinterlegungsnummer

P43431, P43432

Lagertemp.

−20°C

Angaben zum Gen

mouse ... Il12a(16159) , Il12b(16160)

Anwendung

Interleukin-12 (IL-12) from mouse has been used to study the effect of IL-12 on Th1 (type 1 T helper) immunity in Mongolian gerbils (Meriones unguiculatus) infected with Sporothrix schenckii. It has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.

Biochem./physiol. Wirkung

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Aurelio Flores-García et al.
Journal of chemotherapy (Florence, Italy), 27(2), 87-93 (2014-02-20)
Sporotrichosis is a cutaneous fungal infection caused by Sporothrix schenckii. It is known to be mainly contained by Th1 responses. As IL-12 is crucial for Th1 response, we investigated if treatment with recombinant murine IL-12 (rmIL-12) promoted Th1 immunity and/or
Morgane Boulch et al.
Science immunology, 6(57) (2021-03-28)
Chimeric antigen receptor (CAR) T cell therapy relies on the activity of a large pool of tumor-targeting cytotoxic effectors. Whether CAR T cells act autonomously or require interactions with the tumor microenvironment (TME) remains incompletely understood. Here, we report an
Heather M McGee et al.
Immunology and cell biology, 89(7), 767-776 (2011-01-19)
Neonates have a developing immune response, with a predisposition towards induction of tolerance. As the immune system develops, immunity rather than tolerance is induced, with this development of immunity occurring in response to external factors such as the environment. As

Artikel

Interleukin Family (IL)

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.