I5896

Interleukin-7 human

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): IL-7
• MDL-Nummer: MFCD00163948
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤0.500 ng/mL EC₅₀
• Mol-Gew.: 25 kDa
• Verpackung: pkg of 5 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• Farbe: white
• Löslichkeit: water: soluble 0.005-0.010 mL
• UniProt-Hinterlegungsnummer: P13232
• Lagertemp.: −20°C
• Angaben zum Gen: human ... IL7(3574)

Beschreibung

Allgemeine Beschreibung

The interleukin-7 human (IL-7) gene is mapped to human chromosome 8q21.13. It is a non-hematopoietic cell-derived cytokine. IL-7 is majorly produced by the fibroblastic stromal cells, dendritic cells, thymus epithelial cells and skin keratinocytes. Structurally, IL-7 takes up an up-up-down-down 4-helix bundle topology and has two crossover loops.

Biochem./physiol. Wirkung

IL-7 helix A is crucial for its interaction with the interleukin-7 human receptor (IL-7Rα). It is reported to enhance immunity during viral infections and may be useful in cancer immunotherapy. Mutation in the IL-7 gene is implicated in CD4⁺ T-cell lymphopenia.

Interleukin-7 is a lymphoid cell growth factor which affects pre-B, pro-B and early T cells. It was first isolated by Namen in 1988. IL-7 has a number of known activities. It supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic for thymocytes and co-mitogenic with PHA and Con A. IL-7 also stimulates the proliferation of CD4/CD8 cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL-2, IL-4, and IL-6. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. Antibodies against IL-2 affect its activity, suggesting that it functions through IL-2 production. Human natural IL-7 is 25 kDa glycoprotein that has 6 cysteine residues which are important for biological activity. Human and mouse IL-7 have 60% amino acid sequence homology.

Lymphoid cell growth factor which affects pre-B, pro-B and early T cells; supports the growth of early B cells and is mitogenic for thymocytes and co-mitogenic with PHA and Con A; stimulates the proliferation of CD4/CD8 cells.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 10 mM acetic acid containing 250 μg bovine serum albumin.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)