Direkt zum Inhalt
Merck
Alle Fotos(1)

Key Documents

GERPN226

cGMP, Biotrak™ EIA System

Cytiva RPN226

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
41116126
NACRES:
NA.32

Haltbarkeit

Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hersteller/Markenname

Cytiva RPN226

Verpackung

pkg of 96 wells

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

EIAs and ELISAs are convenient, high-sensitivity, peroxidase-based technologies that enable the amount of protein present in a sample to be measured in a single day. Novel lysis reagents have been developed (Lysis Reagents 1 and 2) to facilitate simple and rapid extraction of cellular molecules.

The Biotrak™ cGMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cGMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cGMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.

Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular cAMP. Lysis Reagent 2 sequesters the key component in Lysis Reagent 1 and ensures cAMP is free for subsequent analysis. The detergent/sequestrant complex does not interfere with antigen: antibody binding. Lysis Reagent 1 is simply added to cultured cells, followed by a 5 to 10 min incubation before assay. The antiserum is reconstituted with Lysis Reagent 2. The assay is based on competition between unlabeled cAMP and a fixed quantity of peroxidase-labeled cAMP, for a limited number of binding sites on a cAMP specific antibody.

Anwendung

cGMP, Biotrak™ EIA System has been used for measuring total cellular cyclic GMP (cGMP) or cyclic AMP (cAMP) levels.

Leistungsmerkmale und Vorteile

  • Elimination of inconvenient, time-consuming extraction procedures.
  • Flexible method-choice of assay protocols.
  • Dual range 2–512 fmol/well (acetylation protocol), dual range 50–12 800 fmol/well (non-acetylation protocol).
  • Rapid assay protocol.
  • Nonradioactive.
  • Specific for cGMP
  • Precise and accurate measurement.
  • Ready-to-use substrate.
  • Colour coded reagents

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Biotrak is a trademark of Cytiva

Piktogramme

Corrosion
GHS05

Signalwort

Danger

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Ca2+ -permeable AMPA (-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid) Receptors and Dopamine D1 Receptors Regulate GluA1 Trafficking in Striatal Neurons
David S T and Edward B Z
The Journal of Biological Chemistry, 288(9) (2013)
YC-1 potentiates the nitric oxide/cyclic GMP pathway in corpus cavernosum and facilitates penile erection in rats.
Gin CH, et al.
European Journal of Pharmacology, 458(1-2), 183-189 (2003)

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.