GE28-9893-33
HiLoad® 16/600 Superdex® 75 pg
Cytiva 28-9893-33, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size
Synonym(e):
HiLoad 16/600
About This Item
Empfohlene Produkte
Materialien
bed support (polyamide)
glass column
glass fiber (reinforced)
Mol-Gew.
3000-70000 Da (globular proteins)
Verpackung
pkg of 1 ea
Hersteller/Markenname
Cytiva 28-9893-33
Parameter
<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)
Bettvolumen
120 mL
Säule L × ID
60 cm × 16 mm
Matrix
agarose-dextran composite
dextran-agarose particle platform
polymeric
Aktive Matrixgruppe
dextran-agarose phase
dextran-agarose phase
Durchschnittliche Teilgröße
34 μm
cleaning
1-14
Arbeitsbereich
3-12
Trenntechnik
size exclusion (SEC)
Allgemeine Beschreibung
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Leistungsmerkmale und Vorteile
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
Eignung
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
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Artikel
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Protokolle
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
This page shows how to refold proteins using gel filtration and products from Cytiva.
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
Verwandter Inhalt
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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