Direkt zum Inhalt
Merck

GE28-9893-33

Sigma-Aldrich

HiLoad® 16/600 Superdex® 75 pg

Cytiva 28-9893-33, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

Synonym(e):

HiLoad 16/600

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
41115700
NACRES:
SB.52

Materialien

bed support (polyamide)
glass column
glass fiber (reinforced)

Mol-Gew.

3000-70000 Da (globular proteins)

Verpackung

pkg of 1 ea

Hersteller/Markenname

Cytiva 28-9893-33

Parameter

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

Bettvolumen

120 mL

Säule L × ID

60 cm × 16 mm

Matrix

agarose-dextran composite
dextran-agarose particle platform
polymeric

Aktive Matrixgruppe

dextran-agarose phase
dextran-agarose phase

Durchschnittliche Teilgröße

34 μm

cleaning

1-14

Arbeitsbereich

3-12

Trenntechnik

size exclusion (SEC)

Allgemeine Beschreibung

HiLoad® 16/600 Superdex® 75 prep grade are prepacked 120 mL XK columns designed for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Leistungsmerkmale und Vorteile

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Eignung

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Empfohlene Produkte

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Rechtliche Hinweise

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

Leider sind derzeit keine COAs für dieses Produkt online verfügbar.

Wenn Sie Hilfe benötigen, wenden Sie sich bitte an Kundensupport

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Artikel

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protokolle

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

This page shows how to refold proteins using gel filtration and products from Cytiva.

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Verwandter Inhalt

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.