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GE28-9505-15

HiScreen™ SP High Performance

Cytiva 28-9505-15

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About This Item

UNSPSC-Code:
47101511
NACRES:
NA.56

ligand

sulphopropyl

Beschreibung

Ion Exchanger Type (value)

Verpackung

pkg of 1 ea

Hersteller/Markenname

Cytiva 28-9505-15

Parameter

44 psi

Bettgröße

7.7 mm × 100 mm

Bettvolumen

4.7 mL

Säule ID

7.7 mm

Matrix

6% cross-linked agarose

Partikelgröße

24-44 μm

Durchschnittliche Teilgröße

34 μm

cleaning

3-14

working range

4-13

Eignung

suitable for bioprocess medium

Allgemeine Beschreibung

HiScreen™ SP HP columns are prepacked with SP Sepharose™ High Performance ion exchange chromatography media and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Anwendung

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ IEX HP columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Leistungsmerkmale und Vorteile

  • SP Sepharose™ High Performance is a strong cation exchange BioProcess medium designed for intermediate and polishing steps in downstream processing.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)
Store at 4 to 30 °C (20% Ethanol, 0.2 M Sodium Acetate)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

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Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

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Artikel

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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Protokolle

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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