GE28-9180-08
PD Miditrap G-25
Cytiva 28-9180-08
Synonym(e):
G-25 Miditrap, Miditrap G-25, PD G-25 Miditrap
About This Item
Empfohlene Produkte
Leistungsmerkmale
wetted part: no
Verpackung
pkg of 50 ea (columns)
Hersteller/Markenname
Cytiva 28-9180-08
Lagerbedingungen
dry media
Bettgröße
13 mm × 26 mm
Bettvolumen
3.5 mL
Säule ID
13 mm
Matrix
cross-linked dextran
Partikelgröße
>50 μm (dry)
85-260 μm
cleaning
2-13
working range
2-13
Eignung
suitable for bioprocess medium
Verwandte Kategorien
Allgemeine Beschreibung
PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.
Anwendung
Leistungsmerkmale und Vorteile
- Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
- Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
- High desalting capacity.
- Flexible protocols with ability to use gravity flow or centrifugation.
- Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.
Lagerung und Haltbarkeit
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Analysenzertifikate (COA)
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Artikel
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Protokolle
The emphasis of this page is on the rules and good practice in sample preparation for Western Blots.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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