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GE28-9180-08

PD Miditrap G-25

Cytiva 28-9180-08

Synonym(e):

G-25 Miditrap, Miditrap G-25, PD G-25 Miditrap

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About This Item

UNSPSC-Code:
41115709
NACRES:
NA.56

Leistungsmerkmale

wetted part: no

Verpackung

pkg of 50 ea (columns)

Hersteller/Markenname

Cytiva 28-9180-08

Lagerbedingungen

dry media

Bettgröße

13 mm × 26 mm

Bettvolumen

3.5 mL

Säule ID

13 mm

Matrix

cross-linked dextran

Partikelgröße

>50 μm (dry)
85-260 μm

cleaning

2-13

working range

2-13

Eignung

suitable for bioprocess medium

Allgemeine Beschreibung

PD MidiTrap G-25 is a convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel. Can be used in a centrifugation or gravity protocol.

PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.

Anwendung

Protein sample preparation. Desalting/Buffer Exchange/Cleanup.

Leistungsmerkmale und Vorteile

  • Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
  • Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
  • High desalting capacity.
  • Flexible protocols with ability to use gravity flow or centrifugation.
  • Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2


Analysenzertifikate (COA)

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Artikel

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protokolle

The emphasis of this page is on the rules and good practice in sample preparation for Western Blots.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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