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GE28-4055-00

GST MultiTrap 4B

Cytiva 28-4055-00

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About This Item

UNSPSC-Code:
12161501
NACRES:
NA.32

ligand

glutathione

Materialien

polypropylene and polyethylene

Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hersteller/Markenname

Cytiva 28-4055-00

Verpackung

pkg of 4 x 96-well

Größe

127.8 mm × 85.5 mm × 30.6 mm

Matrix

4% agarose

Partikelgröße

45-165 μm

Durchschnittlicher Durchmesser

90 μm

cleaning

4-13

working range

4-13

Kapazität

>25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)

Verwandte Kategorien

Allgemeine Beschreibung

GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.

Anwendung

GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.

Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.

GST MultiTrap FF is prepacked with Glutathione Sepharose 4 Fast Flow, while GST MultiTrap 4B is prepacked with Glutathione Sepharose 4B.

Leistungsmerkmale und Vorteile

  • 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
  • Highly reproducible well-to well and plate-to-plate results.
  • High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
  • Wells are prepacked with Glutathione Sepharose media for convenience.
  • Easy and predictable scale-up using prepacked GSTrap FF, GSTPrep FF 16/10, and GSTrap 4B columns.

Lagerung und Haltbarkeit

Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

GSTPrep is a trademark of Cytiva
GSTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protokolle

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

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This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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