GE28-4055-00
GST MultiTrap™ 4B
Cytiva 28-4055-00
About This Item
Empfohlene Produkte
ligand
glutathione
Materialien
polypropylene and polyethylene
Haltbarkeit
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Hersteller/Markenname
Cytiva 28-4055-00
Verpackung
pkg of 4 x 96-well
Größe
127.8 mm × 85.5 mm × 30.6 mm
Matrix
4% agarose
Partikelgröße
45-165 μm
Durchschnittlicher Durchmesser
90 μm
cleaning
4-13
working range
4-13
Kapazität
>25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)
Verwandte Kategorien
Allgemeine Beschreibung
Anwendung
Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap™ filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.
GST MultiTrap™ FF is prepacked with Glutathione Sepharose™ 4 Fast Flow, while GST MultiTrap™ 4B is prepacked with Glutathione Sepharose™ 4B.
Leistungsmerkmale und Vorteile
- 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
- Highly reproducible well-to well and plate-to-plate results.
- High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
- Wells are prepacked with Glutathione Sepharose™ media for convenience.
- Easy and predictable scale-up using prepacked GSTrap™ FF, GSTPrep™ FF 16/10, and GSTrap™ 4B columns.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Artikel
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Protokolle
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
This page shows how to solubilize membrane proteins with products from Cytiva.
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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